Sequence-Dependent Pausing of a DNA Repair Helicase
نویسندگان
چکیده
منابع مشابه
Structure of the DNA Repair Helicase XPD
The XPD helicase (Rad3 in Saccharomyces cerevisiae) is a component of transcription factor IIH (TFIIH), which functions in transcription initiation and Nucleotide Excision Repair in eukaryotes, catalyzing DNA duplex opening localized to the transcription start site or site of DNA damage, respectively. XPD has a 5' to 3' polarity and the helicase activity is dependent on an iron-sulfur cluster b...
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Polymerase chain reaction is the most widely used method for in vitro DNA amplification. However, it requires thermocycling to separate two DNA strands. In vivo, DNA is replicated by DNA polymerases with various accessory proteins, including a DNA helicase that acts to separate duplex DNA. We have devised a new in vitro isothermal DNA amplification method by mimicking this in vivo mechanism. He...
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The xeroderma pigmentosum group D (XPD) helicase is a component of the transcription factor IIH complex in eukaryotes and plays an essential role in DNA repair in the nucleotide excision repair pathway. XPD is a 5' to 3' helicase with an essential iron-sulfur cluster. Structural and biochemical studies of the monomeric archaeal XPD homologues have aided a mechanistic understanding of this impor...
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STRUCTURE OF THE DNA REPAIR HELICASE HEL308 REVEALS DNA 1 BINDING AND AUTOINHIBITORY DOMAINS 2 Jodi D Richards†, Kenneth A Johnson †, Huanting Liu, Anne-Marie McRobbie, Stephen McMahon, Muse Oke, 3 Lester Carter, James H Naismith, Malcolm F White 4 Centre for Biomolecular Sciences, University of St Andrews, North Haugh, St Andrews, Fife KY16 9ST. 5 † These authors contributed equally towards th...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2020
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2019.11.2144